In order to improve the pumping performance and mixing effect of valve-less piezoelectric pump, a novel valve-less pump is proposed, which has the characteristics that fish-like composite blocking fluid parts used as the valve, multi-inlet and multi-outlet, and the pump and valve separated. Based on the analysis and comparison of flow resistance unequal between the forward and reverse flow around the fish-like composite blocking fluid parts, the pumping mechanism of the fish-like blocking fluid parts is explained; Through the numerical simulation of the flow field around for the fish-like composite blocking fluid parts, it is revealed that the generation and motion of fluid vortices are the fundamental reason for the high-efficiency mixing of fluids; The working process of fluid transmission driven by pe-riodic vibration of vibrator is analyzed. Then the prototype of valve-less pump is made and its pumping performance is tested. At the driving voltage 180V and the driving frequency 10Hz respectively, the test results are that: 1) the pumping instantaneous flow rate for water is 111.24 mL/min; 2) the pumping in-stantaneous flow rate for mixture fluids is 94.10 mL/min, and a uniform and stable mixture of quality and color is obtained. Theoretical analysis and experimental results show that the valve-less pump with fish-like composite blocking fluid parts can mix and transport fluids, and its mixed transportation per-formance is significantly better than the similar valve-less pumps in the existing literature. It lays a groundwork for the application of valve-less pump in the mixed transportation of small flow fluid.