Determining the acceleration factor which describes the equivalent relation between the time and vibration level is one of the important steps during the vibration durability test. In order to obtain the acceleration factor, based on the law of palmgren-miner fatigue cumulative damage and the theory of cumulative damage under stationary narrow and wide band stress, the calculation equation of acceleration factor is derived. The accelerated life equation, combining with the accelerated life testing theory, is used to design the prototype of the accelerated life test. The test is implemented, and then the test data is verified by weillbull model and processed by least square method, finally the specimen material vibration acceleration factor can be obtained. This test method can provide theoretical and practical guidance to other material acceleration factor test and ambient vibration test.