In order to avoid damaging the optoelectronic theodolite during transportation,the impact on the optoelectronic theodolite and its vibration data are needed at the vehicle design stage.The evaluation method of riding comfort for vehicle was introduced,and an experiment of riding comfort was carried out on an independent suspension vehicle for carrying the optoelectronic theodolite.Three key points on the theodolite were selected according to the structure characteristics of optoelectronic theodolite and acceleration under conditions of different road surfaces and driving speeds were obtained.The relationship of vibration strength of different positions and the whole body with the speeds were illustrated by analyzing and summarizing the test data,which provided an experimental basis for designing vehicle of optoelectronic theodolite.