Considering uncertainty of measurement data, a structural physical parameters identification method is proposed. Firstly, a number of uncertainty parameter discrete datum obtained by practical measurement are preliminary screened, the data fitting method is used to establish the membership function of uncertainty parameter; Secondly based on above analysis, though interval analysis of uncertainty parameters processed by cut set method, interval evaluation of uncertainty parameter is obtained; and then the inverse matrix Eigen value method is used to identify structural stiffness matrix; At last, the result shows that by considering uncertainty of the measurement data, this method can be used to identify the structural stiffness, and stiffness intervals are given, it is more suitable for practical projects.