The coupling vibration response of shaft and bearing in different supporting point because of the change of a bearing load on the multi-support rotor system is proposed. In order to study coupling vibration characteristics, the coupling vibration model of multi-support rotor system is established, and both simulation and experiment are provided. In modeling process, not only the oil film coupling effect in each bearing is considered, but also the support coupling force is obtained through the bearing load sensitivity matrix. Under the two conditions that oil film stiffness is constant and dynamic stiffness, rotor system is numerically analyzed and simulated by Runge-Kutta method. Finally the vibration displacement signal is extracted in the 8 support rotor test bed through taking a support point displacement. The results show the vibration response is significantly different in two cases that the bearing location changed in the steady state and in the transient; the analysis result is more reasonable under the condition of the oil film stiffness for dynamic stiffness.