The piezoelectric pump with valve has attracted broad attention and been widely utilized because of the flexibility in design and the blocking of the valve body. In order to overcome negative effect on the opening and closing of the tilt of the valve body caused by lateral force arising from the operation of the single-arm valve of the piezoelectric pump with single-arm spiral shaped valve and effectively take full advantage of slow opening and fast closing”of the valve body of the piezoelectric pump with spiral shaped valve, the two-arm valve body structure with clamped support cantilever and rotational symmetry is constructed firstly and the piezoelectric pump with symmetrical and spiral shaped valve is designed in view of the valve body. And then, the mechanical analysis of the two-arm valve body with clamped support cantilever and rotational symmetry is carried out and the relation of the pump flow is analyzed. Finally, the experimental study on the effect of valve parameters between single-arm valve and two-arm valve with rotational symmetry on the pump parameter is carried out using the actual prototype. The experimental results show that: when the input voltage is 220 V, the frequency is 10 Hz and the arm width of the two-arm valve with rotational symmetry is 0.3mm, the highest pump flow reached 124.2 ml/min. The flow of the two-arm valve-pump with rotational symmetry is 1.25～2.84 times higher than the flow of the single-arm valve-pump. When the arm width of the inlet and outlet of the symmetric valve is different, the pump flow is higher than that of the same arm width. In addition, the pump flow of the valve with bigger arm width of the inlet and smaller arm width of the outlet is greater than that with smaller arm width of the inlet and bigger arm width of the outlet.