In this paper, a pulsed power supply with needle-free syringe is designed. The drive control system of the pulse power supply is 12 V DC voltage supplied by the battery. Moreover, the Buck and push-pull circuit cascade circuit Boost rectifier filter outputs 0~400 V DC adjustable voltage, and the feedback circuit is used to correct the output voltage. Finally a full-bridge inverter circuit is applied to achieve adjustable output frequency and duty cycle. In this case, the output voltage can linearly change in 0~400V with the duty cycle in the instantaneous high voltage pulse signal. The experimental results show that the pulse signal is stable and reliable, and the output ripple is in the range of 0.5% or less, to meet the needle-free injection of piezoelectric power supply requirement.