Payload isolation device is a new design structure of high precision and high stability, which is adapting for the high resolution satellite optical payload with high precision and high stability working environment. It is an urgent problem to be solved that how to validate its characteristic effectively by ground test and quantitatively evaluate its vibration isolation effect. In order to test the modal characteristic and transmissibility characteristic of payload isolation device, a set of micro-vibration experiment system is designed and built, through which effective test data can be obtained. Through comparison and analysis, the vibration isolation effect of payload isolation device is quantified. The experimental investigation result provides important basic data and valuable engineering experience for the development of payload isolation device, and provides a wide range of reference value for the vibration isolation system design of satellite.