In order to find a simplified calculation method for the vibration frequency of the corrugated web steel box-concrete composite girder bridge， firstly， based on the comprehensive consideration of the shear lag effect of the box girder and the influence of the shear deformation of the corrugated steel web， the element stiffness matrix of the corrugated web steel box-concrete composite girder bridge is derived by the potential energy variation principle. Secondly， according to the derived element stiffness matrix and element mass matrix， the Matlab software is used to compile the calculation program of the vibration frequency calculation of the corrugated web steel box-concrete composite girder bridge. Finally， the convergence speed and computational efficiency of the vibration frequency solving program are analyzed. The results show that the frequency value obtained by the vibration frequency solving program agrees well with the ANSYS spatial finite element value and the measured value. The calculation of the frequency by the vibration frequency solver requires only a small number of units to achieve high calculation accuracy. The calculation time of the vibration frequency is greatly shortened， the calculation efficiency is improved， the complexity of the establishment and solution of the ANSYS spatial finite element model is avoided， and a simple method for analyzing the natural vibration characteristics of this type bridge in the engineering is provided.