In order to study the vibration characteristics of sinking metro depot caused by train operation， one of the largest sinking metro depots in China is taken as the research object. Based on the field measured data， the vibration source characteristics and vibration propagation law of sinking metro depot service shop are analyzed. The results show that when the train runs at a speed of about 9~14 km/h in the service shop， the peak value of rail vibration acceleration in metro deport service shop is about 12.7 m/s2 in the underground negative layer， 2.7 m/s2 in the underground negative second layer. The supported column acceleration is about 0.6 m/s2 in the underground negative layer， 0.2 m/s2 in the underground negative second layer， and the main frequency band is about 100~200 Hz. The vibration of the upper platform caused by train operation decreases linearly with the increase of the distance from the center line of the traveling track. The linear attenuation rate is about 0.2 dB/m at the end of the traveling track and 0.1 dB/m at the middle traveling track， and it decreases monotonously in the frequency range of 50~200 Hz， while it decreases slowly in the low frequency band within 10 Hz. When the train running from underground negative layer， the vibration of the platform slab attenuates about 6.2 dB relative to the first floor of the underground， but its vibration value is close to the limit of the relevant national standards. It is necessary to pay more attention to the vibration of the upper building caused by the negative underground floor. The research results of this paper can provide reference for vibration prediction， vibration comfort evaluation and vibration reduction design of sinking metro depot.