The vibration characteristics of the hydraulic cylinder in the hoisting system of the segment assembling machine have a direct impact on the segment assembly accuracy. The dynamic model of the lifting system of segment assembling machine is established considering hydraulic spring force and hydraulic friction force. The vibration characteristics of the lifting system of segment assembling machine are simulated numerically， and the vibration of the choke of segment assembling machine in assembling different positions is analyzed. In addition， the characteristics are tested and the vibration suppression method of the hoisting system of the assembler is put forward. The results show that when assembling segments in different positions， there is equal amplitude periodic vibration in the assembly machine choke. The vibration speed of the assembly machine's choke is 8~35 mm/s and the vibration displacement amplitude is 0.06~0.3 mm. The vibration displacement of the choke of the assembly machine is 0.2 mm and 0.4 mm when assembling the standard blocks A1 and A2， respectively， which are in accordance with the simulation results. The stiffness of the hydraulic spring can be improved by changing the diameter of the rod less and rod cavity of the hydraulic cylinder. When the diameter of the rod less and rod cavity is 140 mm and 70 mm， respectively， the vibration of the choke will be reduced. However， there is still periodic vibration with high frequency and equal amplitude in the system. Thus， it is necessary to improve the performance of the system by optimizing the hydraulic control system. The research results provide a theoretical reference for the optimal design and vibration suppression of segment assembling machine hoisting systems.