Based on the vehicle-bridge coupling vibration theory of highway bridges， through precise measurement of vehicle-bridge model parameters， vehicle-bridge test models and finite element models are established， and highway bridge vehicle-bridge coupling vibration test systems are designed. The analysis of the influencing factors of the vehicle-bridge coupling vibration test is carried out， including the discussion of the location of the traffic lane， the mass ratio of the vehicle-bridge， and the form of the bridge support， etc. The results show that the experimental value of the natural frequency of the vehicle-bridge model approximately agrees with the theoretical ones， which verifies the applicability of the model； The lateral displacement of 10 cm in the different lane positions has little effect on the dynamic response， and the lane can be selected according to the actual needs in the test； The vehicle-bridge mass ratio is an important factor in the vehicle-bridge dynamic response. In the test， the vehicle-bridge mass ratio can be selected within the range of 0.10 to 0.16 to obtain a more obvious vehicle-bridge vibration response； The vertical acceleration response of the vehicle and the bridge is obvious under the support model 1 （one end of pad bearing and the other of roller bearing）， which can be recommended to select for the bridge support in the test.